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熒光標記蛋白

近年來,流式細胞術被廣泛應用于生物藥研發和生產的各個環節。熒光標記蛋白作為流式檢測的首選試劑,因其預先標記了熒光素基團,可通過一步染色完成檢測,既簡化了檢測流程、節省了檢測時間,又避免了因使用二抗而產生的非特異背景。然而,高質量熒光標記蛋白的生產面臨著諸多難題,如標記后蛋白活性難以維持、產品的批間一致性難以控制等,目前市場上少有高質量熒光標記蛋白可供購買。

ACROBiosystems作為專注于生物藥研發領域的蛋白供應商,利用專業的蛋白研發及標記平臺、穩定株開發平臺和流式細胞分析平臺,開發了一系列包括FITC標記和PE標記的靶點蛋白,適用于基于流式細胞術的分析試驗,如CAR陽性表達率的檢測和流式細胞術篩選抗體候選藥物等。

產品特色
  1. 標記后蛋白活性經流式細胞術驗證
  2. 可提供配套流式檢測protocol供參考
  3. 標記前后活性保持一致
  4. 高批間一致性
應用案例
流式細胞術檢測Anti-CD19 CAR陽性表達率
FACS Analysis of Anti-CD19 CAR Expression
293 cells were transfected with anti-CD19-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251, 10 μg/ml) and C. FITC-labeled protein control. A. Nin-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251) (QC tested).
Protocol
基于流式細胞術的配體結合試驗
Flow Cytometry assay shows that PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) can bind to 293T cells overexpressing human PD-L1. 1μl stock solution per million cells (QC tested).
Protocol
基于流式細胞術的中和抗體篩選試驗
FACS analysis shows that the binding of PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat.No.PD1-HP2F2) to 293T overexpressing PD-L1 was inhibited by increasing concentration of neutralizing antihuman PD-L1 antibody. The IC50 is 0.29 μg/ml (QC tested).
Protocol
質控
高批間一致性
Binding activity of three different lots of FITC-labeled Human MSLN, Fc Tag was evaluated in the above FACS analysis against Anti-MSLN CAR-293 cells. The result shows very high batch-to-batch consistency.
Binding activity of three different lots of FITC-labeled Human MSLN, Fc Tag was evaluated in the above ELISA analysis against Anti-MSLN Antibody. The result shows very high batch-to-batch consistency.
標記前后活性保持一致
Binding activity of the Human MSLN, Fc Tag before and after FITC labeling was evaluated in the above ELISA analysis. The result shows that FITC-Labeled and Unconjugated Human MSLN, Fc Tag have almost the same level of binding activity.
穩定性好
Binding activity of FITC-labeled Human MSLN, Fc Tag was evaluated in the above FACS analysis against Anti-MSLN CAR-293 cells. The result shows that the FITC-labeled Human MSLN is stable at 37℃ for 24 hours without performance reduction.
Binding activity of FITC-labeled Human MSLN, Fc Tag was evaluated in the above FACS analysis against Anti-MSLN CAR-293 cells. The result shows that the FITC-labeled Human MSLN is stable within three freeze-thaw cycles without performance reduction.
產品列表
PE Labeled Protein
Molecule Cat. No. Species Product Description Host Structure
SIRP alpha SIA-HP252 Human PE-Labeled Human SIRP alpha, Fc Tag
(recommended for neutralizing assay)
HEK293
PD-1 PD1-HP2F2 Human PE-Labeled Human PD-1, Fc Tag, His Tag
(recommended for neutralizing assay)
HEK293
Mesothelin MSN-HP223 Human PE-Labeled Human Mesothelin (296-580) HEK293
FITC Labeled Protein
Molecule Cat. No. Species Product Description Host Structure
Mesothelin MSN-HF25x Human FITC-Labeled Human Mesothelin (296-580), Fc Tag HEK293
MSN-HF223 Human PE-Labeled Human Mesothelin (296-580) HEK293
MSN-HF253 Human FITC-Labeled Human Mesothelin (296-580), Fc Tag HEK293
CD30 CD0-HF2H3 Human FITC-Labeled Human CD30, His Tag HEK293
SLAMF7 SL7-HF2H7 Human FITC-Labeled Human SLAMF7, His Tag HEK293
BCMA BCA-HF2H1 Human FITC-Labeled Human BCMA, His Tag HEK293
BCA-HF254 Human FITC-Labeled Human BCMA, Fc Tag HEK293
Siglec-2 SI2-HF2H6 Human FITC-Labeled Human Siglec-2 HEK293
CD19 CD9-HF251 Human FITC-Labeled Human CD19 (20-291), Fc Tag HEK293
CD9-HF2H2 Human FITC-Labeled Human CD19 (20-291) HEK293
Glypican 3 GP3-HF258 Human FITC-Labeled Human Glypican 3, Fc Tag HEK293
GP3-HF2H1 Human FITC-Labeled Human Glypican 3, His Tag HEK293
BAFF BAF-HF268 Human FITC-Labeled Human BAFF / TNFSF13B / CD257 Protein, Fc Tag HEK293
產品定制

国产视频app 為滿足生物藥研發和生產領域客戶的需求,ACROBiosystems正在積極開發高質量的熒光標記蛋白產品。如果您愿意分享您在這個領域的想法和需求,我們將非常感謝。如果您有任何建議或定制要求,請隨時與我們聯系。

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