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FGL1蛋白:LAG-3免疫治療新配體

背景
FGL1是纖維蛋白原蛋白質家族的成員。正常情況下,FGL1在肝臟低水平表達,但在前列腺癌、黑素瘤等許多類型的癌癥中均高表達。最新研究表明,FGL1是LAG-3的主要配體并具有高親和力,表明FGL1的發現將開啟腫瘤免疫治療新時代,為腫瘤的免疫治療提供新契機。
為滿足市場需求,ACROBiosystems開發了由HEK293表達的多種屬(Human, Mouse, Cyno)和多種標簽(Fc, His)的FGL1重組蛋白,更有AviTagTM生物素標記的FGL1蛋白可以助力于您的藥物開發。ACRO對FGL1蛋白的純度、活性等性能進行了驗證,并可免費提供相應的Protocol。
應用
FGL1產品列表(HEK293表達)
Molecule Cat. No. Host Product Description
FGL1
(HEK293表達)
FG1-H52Hy Human Human FGL1 Protein, His Tag
FG1-H5258 Human Human FGL1 Protein, Fc Tag
FG1-H82F4 Human Biotinylated Human FGL1 Protein, Avitag?, Fc Tag
FG1-C5269 Cynomolgus / Rhesus macaque Cynomolgus / Rhesus macaque FGL1 Protein, Fc Tag
FG1-H82Ey Human Biotinylated Human FGL1 Protein, His,Avitag?
(recommended for biopanning)
FG1-C5250 Cynomolgus / Rhesus macaque Cynomolgus / Rhesus macaque FGL1 Protein, Mouse IgG2a Fc Tag

FGL1被鑒定為LAG-3的主要配體,如果您同時對LAG-3感興趣,可以點擊獲得更多LAG-3產品的相關信息。

活性驗證
FGL1的生物活性經Cell-based assay/ BLI/ ELISA驗證

Immobilized Human LAG-3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) at 10 μg/mL (100 μL/well) can bind Human FGL1, Fc Tag (Cat. No. FG1-H5258) with a linear range of 0.02-0.156 μg/mL.国产视频app

国产视频app Protocol

Serial dilutions of Anti-LAG3, Neutralizing Antibody were added into Human FGL1, Fc Tag (Cat. No. FG1-H5258): Biotinylated Human LAG-3, Mouse IgG2a Fc,Avitag (Cat. No. LA3-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.42303 μg/mL.

Protocol

FACS assay shows that Human FGL1, His Tag (Cat. No. FG1-H52Hy) can bind to 293T cells overexpressing human LAG3. The concentration of Human FGL1 is 20 μg/mL.国产视频app

国产视频app Protocol

Loaded Human LAG-3, Fc Tag (Cat. No. LA3-H5255) on Protein A Biosensor, can bind Human FGL1, His Tag (Cat. No. FG1-H52Hy) with an affinity constant of 13.6 nM as determined in BLI assay (ForteBio Octet Red96e).国产视频app

Protocol

*如果您希望ACRO補充更多FGL1產品的實驗設計和應用數據,請 點擊咨詢 国产视频app

*如果您對ACRO有新品開發建議或者對FGL1蛋白有增項建議,請點擊 開發建議

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