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Siglec-15蛋白 - 經Cell based assay驗證生物活性

背景
Siglec-15是唾液酸結合Ig樣凝集素(Siglecs)家族的一員,在M2型巨噬細胞、髓系細胞(MDSC)、樹突狀細胞、B細胞與破骨細胞中表達。相關研究表明,對于絕大多數PD-L1陰性腫瘤患者中,Siglec-15很可能是主要免疫抑制因子,該蛋白與PD-L1是互斥表達的,即PD-L1和Siglec-15在腫瘤細胞內的表達是此消彼長的。Siglec-15可以持續抑制T細胞的活性,并且表現出了滿足正常化癌癥免疫治療的主要特征,對于那些PD-1/PD-L1治療無效的癌癥患者,Siglec-15可能為他們帶來新的免疫治療方案。
高性能的Siglec-15蛋白更能助力于藥物的研發,ACROBiosystems開發的Siglec-15蛋白活性經Cell based assay /BLI /SPR /ELISA多種技術驗證,并且免費提供相應的protocol,更能幫助您縮短研發周期。
產品特點
Cell based assay/ BLI/ SPR/ ELISA多種技術驗證Siglec-15蛋白活性,并免費提供Protocol

国产视频app Cell based assay

Biotinylated Human Siglec-15, Fc,Avitag (Cat. No. SG5-H82F5) inhibits Anti-CD3-induced proliferation of PBMC. The ED50 for this effect is 0.48-0.57 μg/mL.国产视频app

Protocol

BLI

Loaded Anti-Siglec-15 MAb (Mouse IgG) on AMC Biosensor, can bind Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) with an affinity constant of 59.7 pM as determined in BLI assay (ForteBio Octet Red96e).

Protocol

SPR

Immobilized Neu5Ac(a2-6)GalNAc-PAA-biotin on SA Chip can bind Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) with an affinity constant of 4.49 μM as determined in a SPR assay (Biacore T200).

国产视频app Protocol

ELISA

Immobilized Mouse Human chimeric MAb (5G12, Human IgG1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Siglec-15, Fc,Avitag (Cat. No. SG5-H82F5) with a linear range of 0.4-3 ng/mL.

Protocol

多種屬(Human, Mouse, Cyno)& 多標簽(Fc, His, Avi tag)
Cat. No. Species Product Description
SG5-H52H3 Human Siglec-15 / CD33L3 Protein, His Tag
SG5-H5253 Human Siglec-15 / CD33L3 Protein, Fc Tag
SG5-H82F5 Human Biotinylated Siglec-15 / CD33L3 Protein, Fc, Avitag?
SG5-H82E9 Human Biotinylated Siglec-15 / CD33L3 Protein, His, Avitag? (recommended for biopanning)
SG5-M52H7 Mouse Siglec-15 / CD33L3 Protein, His Tag
SG5-C52H6 Cynomolgus Siglec-15 / CD33L3 Protein, His Tag
SG5-C5253 Cynomolgus Siglec-15 / CD33L3 Protein, His Tag

*如果您希望ACRO補充更多Siglec-15產品的實驗設計和應用數據,請 點擊咨詢 国产视频app

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