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CD24 & Siglec-10蛋白 – 腫瘤免疫治療熱門靶點

背景
癌細胞表面表達特定的“別吃我”的信號分子(如CD24、CD47、PD-L1、B2M)后會被免疫細胞識別為正常細胞,從而逃避腫瘤微環境中巨噬細胞的吞噬清除。最早發現的“別吃我”信號蛋白是PD-1和CTLA-4,2019年7月發表在Nature的一項研究表明CD24是卵巢癌和三陰乳腺癌細胞表達的另一個“別吃我”信號蛋白,被癌細胞用來保護自己,CD24是開發癌癥免疫療法潛在靶點。
CD24(Cluster of Differentiation 24)是一種高度糖基化的糖基磷脂酰肌醇錨定的細胞表面蛋白。卵巢癌和三陰乳腺癌細胞表面上表達的CD24蛋白可以與免疫細胞上的Siglec-10蛋白相互作用來傳遞免疫抑制性信號,從而抑制破壞性炎癥反應。
目前,市面的CD24蛋白大多都只有與抗體結合的數據,缺少與Siglec-10蛋白結合活性的驗證,而CD24抗體藥研發的關鍵點在于其能夠有效地阻斷CD24與Siglec-10的結合。為了解決這些問題,ACROBiosystems開發了不同標簽的CD24蛋白以及CD24過表達細胞株,并驗證了細胞株與Siglec-10的結合活性。另外還驗證了抗CD24中和抗體SN3可以有效的阻斷Siglec-10與CD24過表達細胞株的結合。
產品列表
Molecule Cat. No. Species Product Description
CD24CHEK-ATP032-HEK293/Human CD24 Stable Cell LineHOT
CD4-H82E9HumanBiotinylated Human CD24 Protein, His,Avitag?
CD4-H82F5HumanBiotinylated Human CD24 Protein, Fc,Avitag?
CD4-H5254HumanHuman CD24 Protein, Fc Tag
CD4-H5257HumanHuman CD24 Protein, Mouse IgG2a Fc Tag
CD4-H52H3HumanHuman CD24 Protein, His Tag
CD4-M52H7MouseMouse CD24 Protein, His Tag
CD4-C5257CynomolgusCynomolgus CD24 Protein, Fc Tag
Siglec-10SI0-HP2E5HumanPE-Labeled Human Siglec-10 Protein, His Tag (recommended for neutralizing assay)HOT
SI0-H82E3HumanBiotinylated Human Siglec-10 Protein, His,Avitag?
SI0-H82F6HumanBiotinylated Human Siglec-10 Protein, Fc,Avitag?
SI0-H5253HumanHuman Siglec-10 Protein, Fc Tag
SI0-H525bHumanHuman Siglec-10 Protein, Mouse IgG2a Fc Tag, low endotoxin
SI0-H52H9HumanHuman Siglec-10 Protein, His Tag
SI0-M52H7MouseMouse Siglec-10 Protein, His Tag
SI0-C52H5CynomolgusCynomolgus Siglec-10 Protein, His Tag
實驗數據
CD24過表達細胞株與Siglec-10的結合驗證
PE Labeled Siglec-10 Protein
Fig.1 FACS analysis of PE-Labeled Human Siglec-10, His Tag (Cat. No. SI0-HP2E5) at 1/10 dilution binding to 293 cells overexpressing Human CD24 Stable Cell Line (Cat. No. CHEK-ATP032).

Protocol

抗CD24抗體(SN3)抑制Siglec-10與CD24過表達細胞株的結合
Inhibition of Anti-CD24 Antibody (SN3)
Fig.2 FACS analysis shows that the binding of PE-Labeled Human Siglec-10, His Tag (Cat. No. SI0-HP2E5) to 293 cells overexpressing Human CD24 Stable Cell Line (Cat. No. CHEK-ATP032) was inhibited by increasing concentration of neutralizing anti-CD24 antibody [SN3]. The IC50 of neutralizing anti-CD24 antibody [SN3] is 3.026 μg/ml.

Protocol

相關研究進展

CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy.

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