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ELISA Assay Kit for Anti-PD-1 h-mAb in Monkey Serum

ID Components Size (96 tests) Size (480 tests)
PK301-213 Human PD-1 15 μg 60 μg
PK302-213 Biotinylated anti-PD-1 antibody 10 μg 10 μg
PK303-213 Streptavidin-HRP 5 μg 25 μg
PK304-213 Anti-PD-1 antibody 30 μg 30 μg

Background/背景

How a therapeutic antibody is metabolized in the body is pertinently relevant to its efficacy. Therefore, pharmacokinetics study is an important part of the drug development.

PD-1 is probably the most targeted molecule in today’s pharmaceutical industry, thanking to the clinical success of Keytruda? and Opdivo?. Many investigational anti-PD-1 mAbs are being developed. There is a growing need for a standard assay that can be used to facilitate the study of their pharmacokinetics.

Assay Principles/原理

The enclosed ELISA assay kit for anti-PD-1 h-mAb in monkey serum is based on an enzyme immunoassay (ELISA) between recombinant PD-1 protein (ECD) and a biotinylated anti-PD-1 monoclonal antibody. The method employs the principle of competitive ELISA, allowing quantification of monoclonal anti-PD-1 antibodies in monkey serum.

The assay only involves the following steps:

1. Coat the plate with human PD-1;

2. Add the mixture of your sample and biotinylated anti-PD-1 antibody;

3. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate;

国产视频app 4. Record the OD readings and analyze the serum concentration.

Reconstitution/重構方法

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Advantages/產品優勢

Wide Coverage

This kit has been tested for multiple investigational PD-1 antibodies, as well as the commercially available Keytruda? and Opdivo?国产视频app. There is no requirement for the species and subtype of the antibody.

Easy Process

国产视频app There is no longer a need to generate anti-drug antibodies for PK studies.

Clean Result

The use of the biotinylated antibody in the competitive ELISA significantly alleviates the issue of background noise issue that often occurs in traditional ELISA method.

Storage/存儲

国产视频app Upon receipt, please store all items at -20℃.

国产视频app After reconstitution, the stock solution should be kept at -80℃.

It is recommended not to freeze thaw more than 3 times.

This product is stable after storage at:

  1. Room temperature (RT) for 1 month in lyophilized state;
  2. -20℃ for 1 year in lyophilized state;
  3. -80℃ for 4 months under sterile conditions after reconstitution.

Typical Data/典型數據


Name Pembrolizumab Nivolumab Therapeutic PD-1 Antibody J, Human IgG4 Therapeutic PD-1 Antibody X, Human IgG4 Therapeutic PD-1 Antibody H, Human IgG4
Detection Range (μg/ml) 0.03125-20 0.03125-20 0.03125-20 0.03125-20 0.03125-20
Sensitivity (μg/ml) 0.15625 0.15625 0.15625 0.15625 0.15625
%Recovery 86-111 90-117 87-104 96-107 101-112

前沿進展

貨號/價格

国产视频appEPC-V1-96tests / ¥3270

EPC-V1-480tests / ¥11740

開發建議
文檔
聯系電話:
400-682-2521(全國)
010-57737274(北京)
021-26136428(上海)
運輸方式
訂單郵箱:
order.cn@acrobiosystems.com
技術支持郵箱:
techsupport@acrobiosystems.com
PD-1靶點信息
英文全稱:Programmed cell death protein 1
中文全稱:細胞程序性死亡-1
種類:Homo sapiens
上市藥物數量:7詳情
臨床藥物數量:77詳情
最高研發階段:批準上市

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