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Cynomolgus / Rhesus macaque PD-L1 / B7-H1 Protein, His Tag

分子別名(Synonym)

PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

表達區間及表達系統(Source)

国产视频app Cynomolgus / Rhesus macaque PD-L1, His Tag (PD1-C52H4) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # G7PSE7-1). In the region Phe 19 - Arg 238, the AA sequence of Cynomolgus and Rhesus macaque PD-L1 are homologus.

Predicted N-terminus: Phe 19

蛋白結構(Molecular Characterization)

Online(Phe 19 - Arg 238) G7PSE7-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 27.1 kDa. The protein migrates as 37-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

純度(Purity)

国产视频app >95% as determined by SDS-PAGE.

制劑(Formulation)

国产视频app Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

国产视频app This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) SDS-PAGE gel

国产视频app Cynomolgus / Rhesus macaque PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) at 1 μg/mL (100 μL/well) can bind Anti-Human PD-L1 MAb with a linear range of 0.4-3 ng/mL (QC tested).

国产视频app Protocol

Biotinylated Human  ELISA

国产视频app Report

活性(Bioactivity)-SPR

Biotinylated Human SPR

Anti-Human PD-L1 MAb captured on CM5 chip via anti-human IgG Fc antibody surface, can bind Cynomolgus Rhesus / macaque PD-L1, His Tag (Cat. No. PD1-C52H4) with an affinity constant of 23.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind Cynomolgus Rhesus / macaque PD-L1, His Tag (Cat. No. PD1-C52H4) with an affinity constant of 19.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

文獻引用(Citations)

  • 国产视频app

    Authors: X Qian, et al

    Journal: US20190330348A1 43769

    Application: ELISA

    Request for Full-text

背景(Background)

国产视频app Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

 

前沿進展

貨號/價格

PD1-C52H4-100ug / ¥3500

国产视频appPD1-C52H4-1mg / ¥19800

開發建議
文檔
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PD-L1靶點信息
英文全稱:Programmed death-ligand 1
中文全稱:程序性死亡配體1
種類:Homo sapiens
上市藥物數量:3詳情
臨床藥物數量:57詳情
最高研發階段:批準上市

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