国产视频appHamster PCSK9, His Tag (PC9-H52E4) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Ser 691 (Accession # G3GTK5).
国产视频appPredicted N-terminus: Gln 30
国产视频appThis protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 72.9 kDa. The protein migrates as 18 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
国产视频appLyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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国产视频appPlease see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
国产视频appFor long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Hamster PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human LDL R, Strep Tag (Cat. No. LDR-H5281) at 5 μg/mL (100 μL/well) can bind Hamster PCSK9, His Tag (Cat. No. PC9-H52E4) with a linear range of 1-16 ng/mL (QC tested).
国产视频appProprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).