搜索
 >  其他蛋白>SARS-CoV-2 S protein RBD >SAD-S35

Monoclonal Neutralizing Antibody Specific for the SARS-CoV-2 Spike Protein RBD

表達區間及表達系統(Source)

Monoclonal Neutralizing Antibody Specific for the SARS-CoV-2 Spike Protein RBD (SAD-S35) is expressed from human 293 cells (HEK293).

亞型(Isotype)

国产视频app IgG1/kappa

特異性(Specificity)

The product is specific for SARS-CoV-2 Spike Protein S1 subunit and its RBD domain.

純度(Purity)

国产视频app >95% as determined by SDS-PAGE.

制劑(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

重構方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

国产视频app For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 years under sterile conditions after reconstitution.
 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Serial dilutions of Monoclonal Neutralizing Antibody Specific for the SARS-CoV-2 Spike Protein RBD (Cat.No. SAD-S35) was detected by SARS-CoV-2 Inhibitor screening Kit (Cat.No. EP-105) with a half maximal inhibitory concentration (IC50) of 1.472 μg/mL (QC tested).

国产视频app Protocol

Biotinylated Human  ELISA

Immobilized SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H4) at 2 μg/mL (100 μL/well) can bind Monoclonal Neutralizing Antibody Specific for the SARS-CoV-2 Spike Protein RBD (Cat. No. SAD-S35) with a linear range of 0.2-1.95 ng/mL (QC tested).

Protocol

背景(Background)

Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, DPP4, CEACAM etc.. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

前沿進展

貨號/價格

国产视频appSAD-S35-100ug / ¥3800

国产视频appSAD-S35-1mg / ¥25000

開發建議
文檔
聯系電話:
400-682-2521(全國)
010-57737274(北京)
021-26136428(上海)
運輸方式
訂單郵箱:
order.cn@acrobiosystems.com
技術支持郵箱:
techsupport@acrobiosystems.com

消息提示

国产视频app請輸入您的聯系方式,再點擊提交!

確定