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Human Her2 / ErbB2 Protein, Strep Tag

分子別名(Synonym)

ERBB2,CD340,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1

表達區間及表達系統(Source)

国产视频appHuman Her2, Strep Tag (HE2-H5287) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).

Predicted N-terminus: Thr 23

蛋白結構(Molecular Characterization)

Online(Thr 23 - Thr 652) P04626-1

This protein carries a twin strep tag at the C-terminus.

国产视频appThe protein has a calculated MW of 72.4 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

純度(Purity)

国产视频app>95% as determined by SDS-PAGE.

制劑(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

国产视频appContact us for customized product form or formulation.

重構方法(Reconstitution)

国产视频appPlease see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

国产视频appFor long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

国产视频appThis product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Human Her2, Strep Tag (Cat. No. HE2-H5287) SDS-PAGE gel

国产视频appHuman Her2, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

国产视频appImmobilized Human Her2, Strep Tag (Cat. No. HE2-H5287) at 0.05 μg/mL (100 μL/well) can bind Herceptin with a linear range of 0.4-3 ng/mL (QC tested).

Protocol

背景(Background)

国产视频appHuman Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .

 

前沿進展

貨號/價格

HE2-H5287-50ug / ¥3500

開發建議
文檔
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Her2靶點信息
英文全稱:Receptor protein-tyrosine kinase erbB-2
中文全稱:受體蛋白酪氨酸激酶 erbB-2
種類:Homo sapiens
上市藥物數量:22詳情
臨床藥物數量:176詳情
最高研發階段:批準上市

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