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Biotinylated Human FcRn / FCGRT&B2M?Heterodimer Protein, Avitag?,His Tag&Strep II Tag (SPR?& BLI verified)

分子別名(Synonym)

国产视频appFcRn,FCGRT & B2M

表達區間及表達系統(Source)

国产视频appBiotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR?& BLI verified) (FCM-H82W4) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1 (FCGRT) & P61769-1 (B2M)).

Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)

蛋白結構(Molecular Characterization)

Online(Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) P55899-1 (FCGRT) & P61769-1 (B2M)

国产视频appBiotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR?& BLI verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?) and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 38 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.

標記(Biotinylation)

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標記度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

內毒素(Endotoxin)

国产视频appLess than 1.0 EU per μg by the LAL method.

純度(Purity)

国产视频app>95% as determined by SDS-PAGE.

制劑(Formulation)

国产视频appLyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

国产视频appThis product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR?& BLI verified) (Cat. No. FCM-H82W4) SDS-PAGE gel

国产视频appBiotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR?& BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

国产视频appBiotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR?& BLI verified) (Cat. No. FCM-H82W4) captured on Biotin CAP- Series S Sensor Chip can bind Herceptin with an affinity constant of 0.369 μM as determined in a SPR assay (Biacore T200) (QC tested).

国产视频app Protocol

Biotinylated Human SPR

国产视频appImmobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR?& BLI verified) (Cat. No. FCM-H82W4 ) on SA Chip can bind Human HSA,His Tag, low endotoxin (Cat. No. HSA-H522a) with an affinity constant of 1.46 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

国产视频app Protocol

Biotinylated Human SPR

Biotinylated?Human?FCGRT&B2M?Heterodimer?Protein,?Avitag,His?Tag&Strep?II?Tag (SPR?& BLI verified)?(Cat.?No.?FCM-H82W4)?captured?on?Biotin?CAP?-?Series?S?sensor?Chip?can?bind?Human?IgG1?Fc,?Tag?Free (Cat.?No.?FCC-H5214)?with?an?affinity?constant?of?0.728?μM?as?determined?in?a?SPR?assay?(Biacore?8K)?(Routinely?tested).

国产视频app Protocol

Biotinylated Human SPR

Biotinylated?Human?FCGRT&B2M?Heterodimer?Protein,?Avitag,His?Tag&Strep?II?Tag (SPR?& BLI verified)?(Cat.?No.?FCM-H82W4)?captured?on?Biotin?CAP?-?Series?S?sensor?Chip?can?bind?Human?IgG2?Fc,?Tag?Free (SPR verified)?(Cat.?No.?IG2-H5206)?with?an?affinity?constant?of?0.113?μM?as?determined?in?a?SPR?assay?(Biacore?8K)?(Routinely?tested).

国产视频app Protocol

Biotinylated Human SPR

Biotinylated?Human?FCGRT&B2M?Heterodimer?Protein,?Avitag,His?Tag&Strep?II?Tag (SPR?& BLI verified)?(Cat.?No.?FCM-H82W4)?captured?on?Biotin?CAP?-?Series?S?sensor?Chip?can?bind?Human?IgG3?Fc,?Tag?Free (SPR verified)?(Cat.?No.?IG3-H5200)?with?an?affinity?constant?of?3.32?μM?as?determined?in?a?SPR?assay?(Biacore?8K)?(Routinely?tested).

Protocol

Biotinylated Human SPR

国产视频appBiotinylated?Human?FCGRT&B2M?Heterodimer?Protein,?Avitag?,His?Tag&Strep?II?Tag?(Cat.?No.?FCM-H82W4)?captured?on?Biotin?CAP?-?Series?S?sensor?Chip?can?bind?Human?IgG4?Fc,?Tag?Free (SPR verified)?(Cat.?No.?IG4-H5205)?with?an?affinity?constant?of?1.06?μM?as?determined?in?a?SPR?assay?(Biacore?8K)?(Routinely?tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

国产视频appBiotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR?& BLI verified) (Cat. No. FCM-H82W4) on SA Biosensor, can bind Herceptin with an affinity constant of 0.22 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

国产视频appFCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

 

前沿進展

貨號/價格

国产视频appFCM-H82W4-25ug / ¥2500

FCM-H82W4-200ug / ¥9800

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