搜索
 >  免疫檢查點蛋白>PD-1 >PD1-H5284

Human PD-1 / PDCD1 Protein, Strep Tag

分子別名(Synonym)

PDCD1,PD1,CD279,SLEB2

表達區間及表達系統(Source)

Human PD-1, Strep Tag (PD1-H5284) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).

国产视频appN-terminal Sequence Analysis: Leu 25

蛋白結構(Molecular Characterization)

Online(Leu 25 - Gln 167) Q15116-1

国产视频appThis protein carries a twin strep tag at the C-terminus.

The protein has a calculated MW of 19.0 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

純度(Purity)

国产视频app>90% as determined by SDS-PAGE.

制劑(Formulation)

国产视频appLyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

国产视频appFor long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

国产视频appThis product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Human PD-1, Strep Tag (Cat. No. PD1-H5284) SDS-PAGE gel

国产视频appHuman PD-1, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human PD-1, Strep Tag (Cat. No. PD1-H5284) at 2 μg/mL (100 μL/well) can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with a linear range of 4-63 ng/mL (QC tested).

Protocol

Biotinylated Human SPR

Immobilized Human PD-1, Strep Tag (Cat. No. PD1-H5284) at 2 μg/mL (100 μL/well) can bind Human PD-L2, Fc Tag (Cat. No. PD2-H5251) with a linear range of 0.4-6 ng/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

国产视频appImmobilized Human PD-1, Strep Tag (Cat. No. PD1-H5284) at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL (Routinely tested).

国产视频app Protocol

背景(Background)

Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

 

前沿進展

貨號/價格

PD1-H5284-25ug / ¥2600

PD1-H5284-250ug / ¥8800

PD1-H5284-1mg / ¥23000

開發建議
文檔
聯系電話:
400-682-2521(全國)
010-57737274(北京)
021-26136428(上海)
運輸方式
訂單郵箱:
order.cn@acrobiosystems.com
技術支持郵箱:
techsupport@acrobiosystems.com
PD-1靶點信息
英文全稱:Programmed cell death protein 1
中文全稱:細胞程序性死亡-1
種類:Homo sapiens
上市藥物數量:7詳情
臨床藥物數量:77詳情
最高研發階段:批準上市

消息提示

国产视频app請輸入您的聯系方式,再點擊提交!

確定