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Biotinylated Human PCSK9 (D374Y) Protein, Avitag?,His Tag

分子別名(Synonym)

PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9

表達區間及表達系統(Source)

国产视频appMABSol? Biotinylated Human PCSK9 (D374Y), Avitag,His Tag (PCY-H82E7) is expressed from human HEK293 cells. It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1 (D374Y)).

Predicted N-terminus: Gln 31

蛋白結構(Molecular Characterization)

Online(Gln 31 - Gln 692) Q8NBP7-1 (D374Y)

国产视频appThis protein carries an Avi tag (Avitag?) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 73.7 kDa. The protein migrates as 16 kDa,18 kDa and 65 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and proteolytic digestion.

The D374Y mutation results in higher affinity of PCSK9 for LDLR.

標記(Biotinylation)

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

內毒素(Endotoxin)

国产视频appLess than 1.0 EU per μg by the LAL method.

純度(Purity)

>95% as determined by reduced SDS-PAGE.

制劑(Formulation)

国产视频appLyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

国产视频appContact us for customized product form or formulation.

重構方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

国产视频appFor long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Biotinylated Human PCSK9 (D374Y), Avitag,His Tag (Cat. No. PCY-H82E7) SDS-PAGE gel

Biotinylated Human PCSK9 (D374Y), Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

国产视频appImmobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PCSK9 (D374Y), Avitag,His Tag (Cat. No. PCY-H82E7) with a linear range of 4-125 ng/mL (QC tested).

国产视频app Protocol

背景(Background)

国产视频appProprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).Especially,the D374Y mutation results in higher affinity of PCSK9 for LDLR.

 

前沿進展

貨號/價格

PCY-H82E7-25ug / ¥3500

PCY-H82E7-200ug / ¥13800

開發建議
文檔
聯系電話:
400-682-2521(全國)
010-57737274(北京)
021-26136428(上海)
運輸方式
訂單郵箱:
order.cn@acrobiosystems.com
技術支持郵箱:
techsupport@acrobiosystems.com
PCSK9靶點信息
英文全稱:Proprotein convertase subtilisin kexin type 9
中文全稱:前蛋白轉化酶枯草桿菌蛋白酶Kexin-9
種類:Homo sapiens
上市藥物數量:2詳情
臨床藥物數量:27詳情
最高研發階段:批準上市

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