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Human PD-L1 / B7-H1 Protein, Fc Tag (HPLC-verified)

分子別名(Synonym)

国产视频appPD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

表達區間及表達系統(Source)

Human PD-L1, Fc Tag (HPLC-verified) (PD1-H5258) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).

Predicted N-terminus: Phe 19

蛋白結構(Molecular Characterization)

Online(Phe 19 - Arg 238) NP_054862.1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 51.3 kDa. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

純度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制劑(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

国产视频appContact us for customized product form or formulation.

重構方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

国产视频appFor long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

国产视频appThis product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) SDS-PAGE gel

Human PD-L1, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) HPLC images

国产视频appThe purity of Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) was greater than 90% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human PD-1, Strep Tag (Cat. No. PD1-H5284) at 2 μg/mL (100 μL/well) can bind Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) with a linear range of 4-63 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Report

Biotinylated Human ELISA

Immobilized Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) at 5 μg/mL (100 μL/well) can bind Anti-PD-L1 MAb with a linear range of 0.2-2 ng/mL (Routinely tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) at 5 μg/mL (100 μL/well) can bind Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

国产视频appSerial dilutions of Anti-PD-L1 Neutralizing Antibody were added into Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258): Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2966 μg/mL (Routinely tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human PD-1, His Tag (HPLC verified) (Cat. No. PD1-H5221) on HIS1K Biosensor, can bind Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) with an affinity constant of 38.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) on Protein A Biosensor, can bind Human B7-1, His Tag (Cat. No. B71-H5228) with an affinity constant of 18 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) with an EC50 of 0.029 μg/mL (Routinely tested).

国产视频app Protocol

 

活性(Bioactivity)-FACS

Biotinylated Human FACS

国产视频appFlow Cytometry assay shows that Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 10 μg/mL (Routinely tested).

Protocol

Biotinylated Human FACS

FACS analysis shows that the binding of Human PD-L1, Fc Tag (HPLC-verified) to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing Anti-PD-L1 antibody. The concentration of PD-L1 used is 10 μg/mL. The IC50 is 12.92 μg/mL (Routinely tested).

国产视频app Protocol

 

文獻引用(Citations)

  • Authors: X Qian, et al

    Journal: 国产视频appUS20190330348A1 43769

    Application: immunization & ELISA

    Request for Full-text

  • Authors: Ganesan A, et al

    Journal: Sci Rep 43704

    Application: SPR

    Request for Full-text

  • Authors: Boohaker RJ, et al.

    Journal: 国产视频appCancer Lett 2018

    Application: BLI

    Request for Full-text

  • Authors: Yam Alice, et al.

    Journal: 国产视频appUS20180142022A1 2018

    Application: ELISA

    Request for Full-text

背景(Background)

Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

 

前沿進展

貨號/價格

PD1-H5258-100ug / ¥2900

PD1-H5258-1mg (500ug X 2) / ¥21000

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PD-L1靶點信息
英文全稱:Programmed death-ligand 1
中文全稱:程序性死亡配體1
種類:Homo sapiens
上市藥物數量:3詳情
臨床藥物數量:57詳情
最高研發階段:批準上市

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