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Human PD-L1 / B7-H1 Protein, Strep Tag

分子別名(Synonym)

PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

表達區間及表達系統(Source)

国产视频appHuman PD-L1, Strep Tag (PD1-H5282) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).

Predicted N-terminus: Phe 19

蛋白結構(Molecular Characterization)

Online(Phe 19 - Arg 238) NP_054862.1

This protein carries a twin strep tag at the C-terminus.

国产视频appThe protein has a calculated MW of 28.2 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

純度(Purity)

国产视频app>95% as determined by SDS-PAGE.

制劑(Formulation)

国产视频appLyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

国产视频appContact us for customized product form or formulation.

重構方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Human PD-L1, Strep Tag (Cat. No. PD1-H5282) SDS-PAGE gel

国产视频appHuman PD-L1, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

国产视频appImmobilized Human PD-L1, Strep Tag (Cat. No. PD1-H5282) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F2) with a linear range of 0.002-0.25 μg/mL (QC tested).

国产视频app Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

国产视频appLoaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind Human PD-L1, Strep Tag (Cat. No. PD1-H5282) with an affinity constant of 0.891 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

国产视频appLoaded Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H5257) on Protein A Biosensor, can bind Human PD-L1, Strep Tag (Cat. No. PD1-H5282) with an affinity constant of 2.2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

国产视频app Protocol

 

背景(Background)

国产视频appProgrammed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

 

前沿進展

貨號/價格

PD1-H5282-25ug / ¥2600

国产视频appPD1-H5282-250ug / ¥8800

PD1-H5282-1mg (250ug X 4) / ¥23000

開發建議
文檔
聯系電話:
400-682-2521(全國)
010-57737274(北京)
021-26136428(上海)
運輸方式
訂單郵箱:
order.cn@acrobiosystems.com
技術支持郵箱:
techsupport@acrobiosystems.com
PD-L1靶點信息
英文全稱:Programmed death-ligand 1
中文全稱:程序性死亡配體1
種類:Homo sapiens
上市藥物數量:3詳情
臨床藥物數量:57詳情
最高研發階段:批準上市

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