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Biotinylated Human PCSK9?Protein,?Avitag?,His?Tag

分子別名(Synonym)

PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9

表達區間及表達系統(Source)

国产视频appMABSol? Biotinylated Human PCSK9,?Avitag,His?Tag (PC9-H82E7) is expressed from human HEK293 cells. It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1).

国产视频appPredicted N-terminus: Gln 31

蛋白結構(Molecular Characterization)

Online(Gln 31 - Gln 692) Q8NBP7-1

This protein carries an Avi tag (Avitag?) at the C-terminus, followed by a polyhistidine tag.

国产视频appThe protein has a calculated MW of 73.7 kDa. The protein migrates as 20 kDa and 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.

標記(Biotinylation)

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標記度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1国产视频app as determined by the HABA assay.

內毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

純度(Purity)

国产视频app>92% as determined by reduced SDS-PAGE.

制劑(Formulation)

国产视频appLyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

国产视频appContact us for customized product form or formulation.

重構方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Biotinylated Human PCSK9,?Avitag,His?Tag (Cat. No. PC9-H82E7) SDS-PAGE gel

国产视频appBiotinylated Human PCSK9,?Avitag,His?Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

国产视频appImmobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PCSK9,?Avitag,His?Tag (Cat. No. PC9-H82E7) with a linear range of 2-31 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Report

Biotinylated Human ELISA

Immobilized Biotinylated Human PCSK9,?Avitag,His?Tag (Cat. No. PC9-H82E7) can bind PCSK9 Mab, Human IgG with a linear range of 0.05-1.5 ng/mL. The biotinylated PCSK9 is coated at 1 μg/mL (100 μL/well) on a streptavidin coated plates (Routinely tested).

Protocol

Biotinylated Human  ELISA

国产视频appImmobilized Human VLDL R, His Tag (Cat. No. VLR-H5227) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PCSK9,?Avitag,His?Tag (Cat. No. PC9-H82E7) with a linear range of 10-156 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-FACS

Biotinylated Human FACS

FACS analysis shows that Biotinylated Human PCSK9,?Avitag,His?Tag (Cat. No. PC9-H82E7) inhibits LDL uptake in HepG2 cells. The EC50 for this effect is 1.502-2.378 μg/mL.

国产视频app Protocol

 

文獻引用(Citations)

背景(Background)

国产视频appProprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

 

前沿進展

貨號/價格

PC9-H82E7-25ug / ¥3500

PC9-H82E7-200ug / ¥13800

開發建議
文檔
聯系電話:
400-682-2521(全國)
010-57737274(北京)
021-26136428(上海)
運輸方式
訂單郵箱:
order.cn@acrobiosystems.com
技術支持郵箱:
techsupport@acrobiosystems.com
PCSK9靶點信息
英文全稱:Proprotein convertase subtilisin kexin type 9
中文全稱:前蛋白轉化酶枯草桿菌蛋白酶Kexin-9
種類:Homo sapiens
上市藥物數量:2詳情
臨床藥物數量:27詳情
最高研發階段:批準上市

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