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Biotinylated Human PD-1 / PDCD1 Protein, Avitag?,His Tag (recommended for biopanning)

分子別名(Synonym)

PDCD1,PD1,CD279,SLEB2

表達區間及表達系統(Source)

Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (PD1-H82E4) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).

Predicted N-terminus: Leu 25

蛋白結構(Molecular Characterization)

Online(Leu 25 - Gln 167) Q15116-1

This protein carries an Avi tag (Avitag?) at the C-terminus, followed by a polyhistidine tag.

国产视频appThe protein has a calculated MW of 18.6 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

應用說明(Application)

国产视频appPD1-H82E4 works best for experiments that test the binding between PD-1 and candidate antibodies, such as biopanning and other relevant assays.

国产视频appThis product is NOT suitable for testing PD1-PDL1 binding. For this type of application, we strongly recommend you to choose PD1-H82F2 as an alternative.

標記(Biotinylation)

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標記度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

內毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

純度(Purity)

>95% as determined by SDS-PAGE.

制劑(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

国产视频appThis product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) SDS-PAGE gel

国产视频appBiotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

国产视频appImmobilized Nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) with a linear range of 0.8-13 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Report

活性(Bioactivity)-BLI

Biotinylated Human BLI

国产视频appLoaded Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) on SA Biosensor, can bind Human PD-L1, His Tag (HPLC verified) (Cat. No. PD1-H5229) with an affinity constant of 2.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

国产视频app Protocol

Biotinylated Human BLI

Loaded Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) on SA Biosensor, can bind Human PD-L2, His Tag (SPR verified) (Cat. No. PD2-H5220) with an affinity constant of 0.6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

文獻引用(Citations)

  • Authors: Son S, et al

    Journal: J Drug Target 43724

    Application: 国产视频appBiopanning & ELISA

    Request for Full-text

  • Authors: Ganesan A, et al

    Journal: Sci Rep 43704

    Application: ELISA

    Request for Full-text

  • Authors: 国产视频appWang J, et al

    Journal: Mabs 43689

    Application: MSD

    Request for Full-text

  • Authors: V Malathong, et al

    Journal: 国产视频appUS20190048022A1 43510

    Application: ELISA

    Request for Full-text

  • Authors: 国产视频appFromm G, et al

    Journal: 国产视频appJ ImmunoTher Cancer 43452

    Application: ELISA

    Request for Full-text

背景(Background)

Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

 

前沿進展

貨號/價格

PD1-H82E4-25ug / ¥2500

PD1-H82E4-200ug / ¥9800

開發建議
文檔
聯系電話:
400-682-2521(全國)
010-57737274(北京)
021-26136428(上海)
運輸方式
訂單郵箱:
order.cn@acrobiosystems.com
技術支持郵箱:
techsupport@acrobiosystems.com
PD-1靶點信息
英文全稱:Programmed cell death protein 1
中文全稱:細胞程序性死亡-1
種類:Homo sapiens
上市藥物數量:7詳情
臨床藥物數量:77詳情
最高研發階段:批準上市

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