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Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, Avitag?,His Tag&Twin-Strep Tag (BLI?verified)

分子別名(Synonym)

国产视频appFcRn,FCGRT & B2M

表達區間及表達系統(Source)

MABSol? Biotinylated Mouse FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (BLI?verified) (FCM-M82W6) is expressed from human HEK293 cells. It contains AA Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q61559-1 (FCGRT) & P01887-1 (B2M)).

国产视频appPredicted N-terminus: Ser 22 (FCGRT) & Ile 21 (B2M)

蛋白結構(Molecular Characterization)

Online(Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) Q61559-1 (FCGRT) & P01887-1 (B2M)

Biotinylated Mouse FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (BLI?verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 14.7 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag?) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 15 kDa (B2M) respectively due to glycosylation.

標記(Biotinylation)

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標記度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

內毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

純度(Purity)

>95% as determined by SDS-PAGE.

制劑(Formulation)

国产视频appLyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構方法(Reconstitution)

国产视频appPlease see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

国产视频appFor long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Biotinylated Mouse FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (BLI?verified) (Cat. No. FCM-M82W6) SDS-PAGE gel

Biotinylated Mouse FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (BLI?verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Mouse FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (BLI?verified) (Cat. No. FCM-M82W6) on SA Biosensor, can bind Herceptin with an affinity constant of 3.66 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Protocol

 

背景(Background)

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

 

前沿進展

貨號/價格

国产视频appFCM-M82W6-25ug / ¥3500

国产视频appFCM-M82W6-200ug / ¥13800

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