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Biotinylated Mouse PCSK9 Protein, Avitag?,His Tag

分子別名(Synonym)

PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9

表達區間及表達系統(Source)

MABSol? Biotinylated Mouse PCSK9, Avitag,His Tag (PC9-M82E1) is expressed from human HEK293 cells. It contains AA Gln 35 - Gln 694 (Accession # AAH38085.1).

国产视频appPredicted N-terminus: Gln 35

蛋白結構(Molecular Characterization)

Online(Gln 35 - Gln 694) AAH38085.1

国产视频appThis protein carries an Avi tag (Avitag?) at the C-terminus, followed by a polyhistidine tag.

国产视频appThe protein has a calculated MW of 74.9 kDa. The protein migrates as 20 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.

標記(Biotinylation)

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

內毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

純度(Purity)

>95% as determined by SDS-PAGE.

制劑(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

国产视频appContact us for customized product form or formulation.

重構方法(Reconstitution)

国产视频appPlease see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

国产视频appThis product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Biotinylated Mouse PCSK9, Avitag,His Tag (Cat. No. PC9-M82E1) SDS-PAGE gel

Biotinylated Mouse PCSK9, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

国产视频appImmobilized Mouse LDL R, His Tag (Cat. No. LDR-M52H8) at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse PCSK9, Avitag,His Tag (Cat. No. PC9-M82E1) with a linear range of 2-16 ng/mL (QC tested).

国产视频app Protocol

背景(Background)

国产视频appProprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

 

前沿進展

貨號/價格

PC9-M82E1-25ug / ¥3500

国产视频appPC9-M82E1-200ug / ¥13800

開發建議
文檔
聯系電話:
400-682-2521(全國)
010-57737274(北京)
021-26136428(上海)
運輸方式
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order.cn@acrobiosystems.com
技術支持郵箱:
techsupport@acrobiosystems.com
PCSK9靶點信息
英文全稱:Proprotein convertase subtilisin kexin type 9
中文全稱:前蛋白轉化酶枯草桿菌蛋白酶Kexin-9
種類:Homo sapiens
上市藥物數量:2詳情
臨床藥物數量:27詳情
最高研發階段:批準上市

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