搜索
 >  免疫檢查點蛋白>PD-L1 >PD1-M5220

Mouse PD-L1 / B7-H1 Protein, His Tag

分子別名(Synonym)

国产视频appPD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

表達區間及表達系統(Source)

国产视频appMouse PD-L1, His Tag (PD1-M5220) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Thr 238 (Accession # NP_068693).

Predicted N-terminus: Phe 19

蛋白結構(Molecular Characterization)

Online(Phe 19 - Thr 238) NP_068693

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內毒素(Endotoxin)

国产视频appLess than 1.0 EU per μg by the LAL method.

純度(Purity)

国产视频app>95% as determined by SDS-PAGE.

制劑(Formulation)

国产视频appLyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

国产视频appThis product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Mouse PD-L1, His Tag (Cat. No. PD1-M5220) SDS-PAGE gel

国产视频appMouse PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

国产视频appCaptured Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) on Protein A Chip can bind Mouse PD-L1, His Tag (Cat. No. PD1-M5220) with an affinity constant of 2.16 μM as determined in SPR assay (Biacore T200) (Routinely tested).

Protocol

Biotinylated Human SPR

Captured Mouse PD-L1, His Tag (Cat. No. PD1-M5220) on CM5 Chip via Anti-His antibody can bind Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) with an affinity constant of 1.37 μM as determined in SPR assay (Biacore T200) (Routinely tested).

国产视频app Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) on ProteinA Biosensor, can bind Mouse PD-L1, His Tag (Cat. No. PD1-M5220) with an affinity constant of 1.5 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

国产视频app Protocol

Biotinylated Human BLI

Report

 

文獻引用(Citations)

  • Authors: 国产视频appX Qian, et al

    Journal: 国产视频appUS20190330348A1 43769

    Application: ELISA

    Request for Full-text

  • Authors: 国产视频appZ Lin, et al

    Journal: Molecular Therapy: Oncolytics 43630

    Application: PD-L1 PD-1 Blockade assay (ELISA)

    Request for Full-text

  • Authors: Wang Aijun, et al.

    Journal: US20170355770A1 2017

    Application: Immunization

    Request for Full-text

背景(Background)

Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

 

前沿進展

貨號/價格

PD1-M5220-100ug / ¥3100

PD1-M5220-1mg / ¥18000

開發建議
文檔
聯系電話:
400-682-2521(全國)
010-57737274(北京)
021-26136428(上海)
運輸方式
訂單郵箱:
order.cn@acrobiosystems.com
技術支持郵箱:
techsupport@acrobiosystems.com
PD-L1靶點信息
英文全稱:Programmed death-ligand 1
中文全稱:程序性死亡配體1
種類:Homo sapiens
上市藥物數量:3詳情
臨床藥物數量:57詳情
最高研發階段:批準上市

消息提示

国产视频app請輸入您的聯系方式,再點擊提交!

確定