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Biotinylated Mouse PD-L1 / B7-H1 Protein, Fc,Avitag?

分子別名(Synonym)

国产视频appPD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

表達區間及表達系統(Source)

MABSol? Biotinylated Mouse PD-L1, Fc,Avitag (PD1-M82F5) is expressed from human HEK293 cells. It contains AA Phe 19 - Thr 238 (Accession # NP_068693).

Predicted N-terminus: Phe 19

蛋白結構(Molecular Characterization)

Online(Phe 19 - Thr 238) NP_068693

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag?).

国产视频appThe protein has a calculated MW of 53.2 kDa. The protein migrates as 65-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

標記(Biotinylation)

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標記度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1国产视频app as determined by the HABA assay.

內毒素(Endotoxin)

国产视频appLess than 1.0 EU per μg by the LAL method.

純度(Purity)

国产视频app>95% as determined by SDS-PAGE.

制劑(Formulation)

国产视频appLyophilized from 0.22 μm filtered solution in Tris?with?Glycine,?Arginine?and?NaCl,?pH7.5. Normally trehalose is added as protectant before lyophilization.

国产视频appContact us for customized product form or formulation.

重構方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

国产视频appFor long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Biotinylated Mouse PD-L1, Fc,Avitag (Cat. No. PD1-M82F5) SDS-PAGE gel

Biotinylated Mouse PD-L1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Mouse PD-L1, Fc,Avitag (Cat. No. PD1-M82F5) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Mouse PD-1, Mouse IgG2a Fc Tag (Cat. No. PD1-M52A4) with a linear range of 5-39 ng/mL (QC tested).

国产视频app Protocol

背景(Background)

Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

 

前沿進展

貨號/價格

PD1-M82F5-25ug / ¥3500

PD1-M82F5-200ug / ¥13800

開發建議
文檔
聯系電話:
400-682-2521(全國)
010-57737274(北京)
021-26136428(上海)
運輸方式
訂單郵箱:
order.cn@acrobiosystems.com
技術支持郵箱:
techsupport@acrobiosystems.com
PD-L1靶點信息
英文全稱:Programmed death-ligand 1
中文全稱:程序性死亡配體1
種類:Homo sapiens
上市藥物數量:3詳情
臨床藥物數量:57詳情
最高研發階段:批準上市

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