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Rat PD-1 / PDCD1 Protein, Fc Tag

分子別名(Synonym)

PDCD1,PD1,CD279,SLEB2

表達區間及表達系統(Source)

国产视频appRat PD-1, Fc Tag (PD1-R5253) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_001100397.1).

国产视频appPredicted N-terminus: Leu 25

蛋白結構(Molecular Characterization)

Online(Leu 25 - Gln 167) NP_001100397.1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 42.9 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE).

內毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

純度(Purity)

>95% as determined by SDS-PAGE.

制劑(Formulation)

国产视频appLyophilized from 0.22 μm filtered solution in Tris?with?Glycine,?Arginine?and?NaCl,?pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

国产视频appThis product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Rat PD-1, Fc Tag (Cat. No. PD1-R5253) SDS-PAGE gel

Rat PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse PD-L2, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. PD2-M52A5) at 2 μg/mL (100 μL/well) can bind Rat PD-1, Fc Tag (Cat. No. PD1-R5253) with a linear range of 2-20 ng/mL (QC tested).

国产视频app Protocol

背景(Background)

国产视频appProgrammed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

 

前沿進展

貨號/價格

PD1-R5253-100ug / ¥2900

PD1-R5253-1mg / ¥18000

開發建議
文檔
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PD-1靶點信息
英文全稱:Programmed cell death protein 1
中文全稱:細胞程序性死亡-1
種類:Homo sapiens
上市藥物數量:7詳情
臨床藥物數量:77詳情
最高研發階段:批準上市

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