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Biotinylated Rat FcRn / FCGRT&B2M Heterodimer Protein, Avitag?,His Tag&Strep II Tag (SPR verified)

分子別名(Synonym)

FcRn,FCGRT & B2M

表達區間及表達系統(Source)

MABSol? Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR verified) (FCM-R82W7) is expressed from human HEK293 cells. It contains AA Ala 23 - Ser 298 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P13599-1 (FCGRT) & P07151-1 (B2M)).

Predicted N-terminus: Ala 23 (FCGRT) & Ile 21 (B2M)

蛋白結構(Molecular Characterization)

Online(Ala 23 - Ser 298 (FCGRT) & Ile 21 - Met 119 (B2M)) P13599-1 (FCGRT) & P07151-1 (B2M)

国产视频appBiotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag?) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 14 kDa (B2M) respectively due to different glycosylation.

標記(Biotinylation)

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標記度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

內毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

純度(Purity)

国产视频app>95% as determined by SDS-PAGE.

制劑(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

国产视频appFor long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR verified) (Cat. No. FCM-R82W7) SDS-PAGE gel

国产视频appBiotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

国产视频appBiotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR verified) (Cat. No. FCM-R82W7) captured on Biotin CAP-Series S Sensor Chip can bind Herceptin? with an affinity constant of 10.3 nM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

 

背景(Background)

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

 

前沿進展

貨號/價格

国产视频appFCM-R82W7-25ug / ¥3500

国产视频appFCM-R82W7-200ug / ¥13800

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