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Rhesus macaque PCSK9 Protein, His Tag

分子別名(Synonym)

PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9

表達區間及表達系統(Source)

国产视频appRhesus macaque PCSK9, His Tag (PC9-C52H2) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # A8T666).

国产视频appPredicted N-terminus: Gln 31

蛋白結構(Molecular Characterization)

Online(Gln 31 - Gln 692) A8T666

国产视频appThis protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 73.2 kDa. The protein migrates as 21 kDa and 64 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

純度(Purity)

>95% as determined by SDS-PAGE.

制劑(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構方法(Reconstitution)

国产视频appPlease see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

電泳(SDS-PAGE)

Rhesus macaque PCSK9, His Tag (Cat. No. PC9-C52H2) SDS-PAGE gel

Rhesus macaque PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

国产视频appImmobilized Human LDL R, Strep Tag (Cat. No. LDR-H5281) at 2 μg/mL (100 μL/well) can bind Rhesus macaque PCSK9, His Tag (Cat. No. PC9-C52H2) with a linear range of 1-16 ng/mL (QC tested).

国产视频app Protocol

背景(Background)

国产视频appProprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene.This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

 

前沿進展

貨號/價格

国产视频appPC9-C52H2-50ug / ¥2600

PC9-C52H2-1mg (250ug X 4) / ¥19800

開發建議
文檔
聯系電話:
400-682-2521(全國)
010-57737274(北京)
021-26136428(上海)
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PCSK9靶點信息
英文全稱:Proprotein convertase subtilisin kexin type 9
中文全稱:前蛋白轉化酶枯草桿菌蛋白酶Kexin-9
種類:Homo sapiens
上市藥物數量:2詳情
臨床藥物數量:27詳情
最高研發階段:批準上市

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